Journal: Cancer Research
Article Title: Megakaryocytes Mediate Hyperglycemia-Induced Tumor Metastasis
doi: 10.1158/0008-5472.can-21-1180
Figure Lengend Snippet: Figure 2. Hyperglycemia boosts platelet activation and clearance invivo. A, Platelets from control or hyperglycemic mice were stimulated with ADP, AA, thrombin, or collagen. Quantifications of the percentages of CD62pþ platelets upon the indicated treatments (n ¼ 6 samples per group). B, Representative aggregometry tracings of platelet-rich plasma in response to ADP in control or hyperglycemic groups. The hyperglycemic group showed increased aggregation (n ¼ 3 samples per group). C, Representative clot retraction micrographs of platelet-rich plasma in response to collagen agonist in control or hyperglycemic groups. Quantifications of the clot retraction analysis (n ¼ 6 samples per group). D, PLT, PDW, MPV, PCT analysis in whole blood from control or hyperglycemic groups (n ¼ 6 samples per group). E and F, Occlusion of mesenteric arterioles upon FeCl3-induced injury of the endothelial barrier. Representative thrombi micrographs upon FeCl3- induced mesenteric arteriole injury with platelets from control or hyperglycemic groups. Quantifications of FeCl3-induced mesenteric arteriole thrombosis (n ¼ 6 samples per group). G, CD42b (green) and F4/80 (red) immunofluorescent staining micrographs of the spleen in control or hyperglycemic mice. Quantifications of CD42bþ area (n ¼ 6 random fields per group). Scale bar in top panels, 100 mm. Scale bar in bottom panels, 50 mm. H, Sham operation or splenectomy was performed and mice were then transferred to hyperglycemia modeling for 7 days. Peripheral blood samples were collected for whole blood analysis. Quantifications of PLT in the indicated groups (n ¼ 6 samples per group). , P < 0.05; , P < 0.01; , P < 0.001; n.s., not significant. Data presented as mean SEM.
Article Snippet: For immunofluorescence staining, paraffin-embedded tissue sections or cells on glass coverslips were stained with a rabbit anti-CD42b antibody (catalog no. 12860-1-AP, Proteintech; RRID:AB_10644481; 1:50); a rabbit anti-GRP75 antibody (catalog no. 3593S, Cell Signaling Technology; RRID:AB_2120328; 1:100), and a rabbit anti-SLC2A1 antibody (catalog no. A11170, ABclonal; RRID:AB_2758445; 1:100).
Techniques: Activation Assay, Control, Clinical Proteomics, Staining
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